High-Sensitivity Fluorometry to Resolve Ion Channel Conformational Dynamics
نویسندگان
چکیده
منابع مشابه
Conformational dynamics of the Na+/K+-ATPase probed by voltage clamp fluorometry.
The method of voltage clamp fluorometry combined with site-directed fluorescence labeling was used to detect local protein motions of the fully active Na(+)K(+)-ATPase in real time under physiological conditions. Because helix M5 extends from the cytoplasmic site of ATP hydrolysis into the cation binding region, we chose the extracellular M5-M6 loop of the sheep alpha(1)-subunit for the inserti...
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ژورنال
عنوان ژورنال: Cell Reports
سال: 2018
ISSN: 2211-1247
DOI: 10.1016/j.celrep.2018.01.029